Isolation and characterization of bioactive components from hydroalcoholic extract of Cymbopogon jwarancusa (Jones) Schult. to evaluate its hepatoprotective activity

ETHNOPHARMACOLOGICAL RELEVANCECymbopogon jwarancusa (Jones) Schult. (Family: Poacea/Gramineae) is being used to treat numerous ailments in traditional/folklore and indigenous system of medicine due to its antioxidant, anti-allergic, antiparasitic, analgesic, antipyretic and anticancer activities; however there is no documented evidence regarding its hepatoprotective efficacy.AIMThis study was aimed to evaluate hepatoprotective activity of hydroalcoholic extract of Cymbopogon jwarancusa (HECJ) against paracetamol (PCM) induced liver damage in albino Wistar rats, and to identify the bioactive components present in the extract responsible for the said activity.MATERIALS AND METHODSFive groups of rats (n = 6) were orally treated with: 0.5% carboxymethyl cellulose (normal control), 50 mg/kg silymarin (reference standard), HECJ [515 mg/kg (low dose) and 720 mg/kg (high dose)] (test groups) for 7 days daily, followed by induction of hepato-toxicity using PCM (2 gm/kg) on 7th day (PCM control; reference standard; test groups). The blood samples to estimate the level of AST, ALT, ALP, total bilirubin and total protein; liver tissue homogenate for antioxidant markers (GSH, GST, GPx, and LPO) and liver section for histopathological analysis were collected. Isolation and characterization of phytochemicals from HECJ was done by preliminary screening, determination of phenolic, flavonoid and terpenoid content, and GC-MS analysis.RESULTSThe animals pre-treated with HECJ dose-dependently and significantly alleviated the PCM-induced alterations in liver enzymes, plasma proteins, serum total bilirubin and antioxidant markers levels. The histopathological analysis suggest that PCM causes marked necrosis and lymphocyte infiltration, while preservation of the normal hepatic architecture was observed in groups pre-treated with, reference standard drug silymarin, and HECJ. Preliminary screening of the extract, determination of phenolic, flavonoid and terpenoid content, and GC-MS analysis revealed the presence of some important bioactive components such as phenolics, flavonoids, glycoside, tannins, steroids, fatty acids, sterols, esters, saponins, terpenes/terpenoids and essential oil which could be synergistically responsible for the plant's hepatoprotective effect.CONCLUSIONSThis study concluded that C. jwarancusa could be taken as a beneficial natural product for its hepatoprotective efficacy; however, future line of work is required to establish its precise mechanism of actio