An experimental approach to determine the gamma radiation interaction mean free path and exposure buildup factor for biomolecules

This experimental approach was designed to understand the gamma interaction parameters for the essential biomolecules, including starch soluble, cholesterol, myristic acid, glucose, oxalic acid, dextrose, salicylic acid, ethyl cellulose and sucrose. The empirical determination of gamma interaction parameters, such as interaction mean-free-path (MFP), buildup factor, and effective atomic number (Zeff) was performed by measuring mass attenuation coefficient (μ/ρ) at energies of 356 keV, 511 keV, 662 keV, 1173 keV, 1275 keV and 1332 keV. This was achieved using weak radioactive sources and a NaI(Tl) scintillation spectrometer with collimated and non-collimated transmission geometry. The experimentally determined values of gamma-ray interaction parameters were obtained non-destructively and precisely agreeing with the expected values from simulations and codes. In addition, the research findings also revealed a novel trend in gamma interaction mean free path as a function of energy and variable buildup factors for the selected biomolecules. These research findings provide valuable insight into the process of gamma radiation interaction. This approach may fulfil the increasing demand of medical, technical and academic research laboratories for a cost-effective and reliable empirical methodology to understand gamma radiation interaction with matter. •The gamma ray buildup factor for essential biomolecules was experimentally determined.•The gamma ray interaction mean-free-path was de-convoluted through empirical buildup factor.•An extended elemental calibration curve is provided to determine the μ/ρ of any elemental foil (6 ≤ Z ≤ 83).•The present experiment was carried out with weak radioactive sources and a NaI(Tl) gamma ray scintillation spectrometer.•Experimental approach using the transmission geometry has been validated with theoretical values from standard methods.