Interhelical E@g‐N@a interactions modulate coiled coil stability within a de novo set of orthogonal peptide heterodimers

The designability of orthogonal coiled coil (CC) dimers, which draw on well‐established design rules, plays a pivotal role in fueling the development of CCs as synthetically versatile assembly‐directing motifs for the fabrication of bionanomaterials. Here, we aim to expand the synthetic CC toolkit through establishing a “minimalistic” set of orthogonal, de novo CC peptides that comprise 3.5 heptads in length and a single buried Asn to prescribe dimer formation. The designed sequences display excellent partner fidelity, confirmed via circular dichroism (CD) spectroscopy and Ni‐NTA binding assays, and are corroborated in silico using molecular dynamics (MD) simulation. Detailed analysis of the MD conformational data highlights the importance of interhelical E@g‐N@a interactions in coordinating an extensive 6‐residue hydrogen bonding network that “locks” the interchain Asn‐Asn′ contact in place. The enhanced stability imparted to the Asn‐Asn′ bond elicits an increase in thermal stability of CCs up to ~15°C and accounts for significant differences in stability within the collection of similarly designed orthogonal CC pairs. The presented work underlines the utility of MD simulation as a tool for constructing de novo, orthogonal CCs, and presents an alternative handle for modulating the stability of orthogonal CCs via tuning the number of interhelical E@g‐N@a contacts. Expansion of CC design rules is a key ingredient for guiding the design and assembly of more complex, intricate CC‐based architectures for tackling a variety of challenges within the fields of nanomedicine and bionanotechnology. A set of orthogonal, de novo coiled coil peptides are established comprising 3.5 heptads in length and a single buried Asn to prescribe dimer formation. The designed sequences display excellent partner fidelity. Interhelical E@g‐N@a interactions coordinate an extensive 6‐residue hydrogen bonding network that keeps the interchain Asn‐Asn′ contact in place.