Cx43 regulates mechanotransduction mechanisms in human preterm amniotic membrane defects

What's already known about this topic? In term amniotic membrane (AM), mechanical stimulation increased Cx43 plaque formation that prevents migration and healing by amniotic mesenchymal cells (AMCs). Cx43 also increased myofibroblast migration to the wound edge, resulting in cell/collagen contraction, healing and closure of the defect site. What does this study add? In preterm AM defects, mechanical stimulation increased cell deformation in the fibroblast layer and collagen polarisation around the wound edge. Mechanical stimulation increased Cx43 in myofibroblasts and amniotic epithelial cells, but this response was dependent on tissue region. Markers for migration, inflammation and repair were influenced by mechanical stimulation.