N-substituting perturbation on the interaction affinity and recognition specificity between rheumatic immune-related Abl SH3 domain and its peptoid ligands

Abl is a nonreceptor tyrosine kinase involved in a variety of disease pathways such as rheumatic immune. Full-length Abl protein consists of a catalytic tyrosine kinase (TK) domain as well as two regulatory Src homology domains 2 and 3 (SH2 and SH3, respectively); the latter recognizes and binds to...

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Veröffentlicht in:Journal of molecular graphics & modelling 2023-12, Vol.125, p.108601-108601, Article 108601
Hauptverfasser: Tang, Xiaomin, Chen, Jingjin, Cai, Jiahui, Wang, Qiuqin
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Sprache:eng
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Zusammenfassung:Abl is a nonreceptor tyrosine kinase involved in a variety of disease pathways such as rheumatic immune. Full-length Abl protein consists of a catalytic tyrosine kinase (TK) domain as well as two regulatory Src homology domains 2 and 3 (SH2 and SH3, respectively); the latter recognizes and binds to those natural proline-rich peptide segments containing a PxxP motif on the protein surface of its interacting partners. However, natural peptides cannot bind effectively to the modular domain in high affinity and strong selectivity due to their small size and broad specificity. Here, a synthetic proline-rich peptide p41 was used as template; its structural diversity was extended by combinationally replacing the Pro0 and Pro+3 residues with a number of N-substituted amino acids. Consequently, peptide affinity change upon the replacement was derived to create a systematic N-substituting perturbation profile, from which we identified several N-substitution combinations at the Pro0 and Pro+3 residues of p41 PxxP motif that may moderately or significantly improve the peptide binding potency to Abl; they represent potent peptoid binders of Abl SH3 domain, with affinity improved considerably relative to p41. More significantly, the designed potent peptoids were also found to exhibit a good SH3-selectivity for their cognate Abl over other noncognate nonreceptor tyrosine kinases, with S = 9.7-fold. [Display omitted] •The structural diversity of proline-rich peptide is extended by using N-substituted amino acids.•A systematic profile is created to cover N-substituting perturbation effect on peptide binding.•N-substitution can be used to derive a number of the promising peptoid ligands of Abl SH3 domain.•Both the affinity and specificity of peptoids are improved relative to proline-rich peptide.
ISSN:1093-3263
1873-4243
DOI:10.1016/j.jmgm.2023.108601