Microencapsulation of parathyroid cells via electric field and non-surgical transplantation approach
Purpose Hypoparathyroidism is a rare disease with low PTH, mostly seen as a consequence of neck surgery. Current management is the prescription of calcium and vitamin D, but the definitive treatment is parathyroid allotransplantation, which frequently triggers an immune response, thus cannot achieve...
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| Veröffentlicht in: | Journal of endocrinological investigation 2023-11, Vol.46 (11), p.2257-2267 |
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| creator | Karabıyık Acar, Ö. Başoğlu, H. Keğin, M. Nozhatzadeh, G. D. Hacıhasanoğlu, E. Tuncer, A. A. Şahin, F. Torun Köse, G. Aysan, E. |
| description | Purpose
Hypoparathyroidism is a rare disease with low PTH, mostly seen as a consequence of neck surgery. Current management is the prescription of calcium and vitamin D, but the definitive treatment is parathyroid allotransplantation, which frequently triggers an immune response, thus cannot achieve the expected success. To overcome this problem, encapsulation of allogeneic cells is the most promising method. By optimizing the standard alginate cell encapsulation technique with parathyroid cells under high-voltage application, the authors reduced the size of parathyroid-encapsulated beads and evaluated these samples in vitro and in vivo
.
Methods
Parathyroid cells were isolated, and standard-sized alginate macrobeads were prepared without any electrical field application, while microbeads in smaller sizes ( |
| doi_str_mv | 10.1007/s40618-023-02075-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2854433776</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2854433776</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-735ece8637b2f5a1af13b98b3e23d93b5c568144435a8821f19b7bcdececde213</originalsourceid><addsrcrecordid>eNp9kUtLxTAQhYMoXl9_wIUE3LipJp2myV3KxRdccaPrME1TzaU3rUkr-O-N1hcuXCQZyHfODHMIOeTslDMmz2LBSq4ylkM6TIpMbpAdLnOWKVDl5q96RnZjXDEGEpTcJjMo57IsVLFD6ltnQme9wT6OLQ6u87RraI8Bh6fX0LmaGtu2kb44pLa1ZgjO0MbZtqboa-o7n8UxPDqDLR0C-ti36IfJCPs-dGie9slWg220B5_vHnm4vLhfXGfLu6ubxfkyMyDFkEkQ1lhVgqzyRiDHhkM1VxXYHOo5VMKIUvGiKECgUjlv-LySlamTKF05hz1yMvmmts-jjYNeu_g-PnrbjVHnSiQxSFkm9PgPuurG4NN0iZIgVSEKlqh8otKOYgy20X1wawyvmjP9noGeMtApA_2RgZZJdPRpPVZrW39LvpaeAJiAmL78ow0_vf-xfQN7uZNh</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2873784540</pqid></control><display><type>article</type><title>Microencapsulation of parathyroid cells via electric field and non-surgical transplantation approach</title><source>Springer Nature - Complete Springer Journals</source><creator>Karabıyık Acar, Ö. ; Başoğlu, H. ; Keğin, M. ; Nozhatzadeh, G. D. ; Hacıhasanoğlu, E. ; Tuncer, A. A. ; Şahin, F. ; Torun Köse, G. ; Aysan, E.</creator><creatorcontrib>Karabıyık Acar, Ö. ; Başoğlu, H. ; Keğin, M. ; Nozhatzadeh, G. D. ; Hacıhasanoğlu, E. ; Tuncer, A. A. ; Şahin, F. ; Torun Köse, G. ; Aysan, E.</creatorcontrib><description>Purpose
Hypoparathyroidism is a rare disease with low PTH, mostly seen as a consequence of neck surgery. Current management is the prescription of calcium and vitamin D, but the definitive treatment is parathyroid allotransplantation, which frequently triggers an immune response, thus cannot achieve the expected success. To overcome this problem, encapsulation of allogeneic cells is the most promising method. By optimizing the standard alginate cell encapsulation technique with parathyroid cells under high-voltage application, the authors reduced the size of parathyroid-encapsulated beads and evaluated these samples in vitro and in vivo
.
Methods
Parathyroid cells were isolated, and standard-sized alginate macrobeads were prepared without any electrical field application, while microbeads in smaller sizes (< 500 µm), by the application of 13 kV. Bead morphologies, cell viability, and PTH secretion were evaluated in vitro for four weeks. For the in vivo part, beads were transplanted into Sprague–Dawley rats, and after retrieval, immunohistochemistry and PTH release were evaluated in addition to the assessment of cytokine/chemokine levels.
Results
The viability of parathyroid cells in micro- and macrobeads did not differ significantly. However, the amount of in vitro PTH secretion from microencapsulated cells was significantly lower than that from macroencapsulated cells, although it increased throughout the incubation period. Immunohistochemistry of PTH staining in both of the encapsulated cells identified as positive after retrieval.
Conclusion
Contrary to the literature, a minimal in vivo immune response was developed for alginate-encapsulated parathyroid cells, regardless of bead size. Our findings suggest that injectable, micro-sized beads obtained using high-voltage may be a promising method for a non-surgical transplantation approach.
Graphical abstract</description><identifier>ISSN: 1720-8386</identifier><identifier>ISSN: 0391-4097</identifier><identifier>EISSN: 1720-8386</identifier><identifier>DOI: 10.1007/s40618-023-02075-7</identifier><identifier>PMID: 36976484</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Alginic acid ; Cell size ; Cell viability ; Chemokines ; Encapsulation ; Endocrinology ; Hypoparathyroidism ; Immune response ; Immunohistochemistry ; Internal Medicine ; Medicine ; Medicine & Public Health ; Metabolic Diseases ; Microencapsulation ; Microspheres ; Original Article ; Parathyroid hormone ; Transplantation ; Vitamin D ; Voltage</subject><ispartof>Journal of endocrinological investigation, 2023-11, Vol.46 (11), p.2257-2267</ispartof><rights>The Author(s), under exclusive licence to Italian Society of Endocrinology (SIE) 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Italian Society of Endocrinology (SIE).</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-735ece8637b2f5a1af13b98b3e23d93b5c568144435a8821f19b7bcdececde213</citedby><cites>FETCH-LOGICAL-c375t-735ece8637b2f5a1af13b98b3e23d93b5c568144435a8821f19b7bcdececde213</cites><orcidid>0000-0003-2697-6477</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s40618-023-02075-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s40618-023-02075-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36976484$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Karabıyık Acar, Ö.</creatorcontrib><creatorcontrib>Başoğlu, H.</creatorcontrib><creatorcontrib>Keğin, M.</creatorcontrib><creatorcontrib>Nozhatzadeh, G. D.</creatorcontrib><creatorcontrib>Hacıhasanoğlu, E.</creatorcontrib><creatorcontrib>Tuncer, A. A.</creatorcontrib><creatorcontrib>Şahin, F.</creatorcontrib><creatorcontrib>Torun Köse, G.</creatorcontrib><creatorcontrib>Aysan, E.</creatorcontrib><title>Microencapsulation of parathyroid cells via electric field and non-surgical transplantation approach</title><title>Journal of endocrinological investigation</title><addtitle>J Endocrinol Invest</addtitle><addtitle>J Endocrinol Invest</addtitle><description>Purpose
Hypoparathyroidism is a rare disease with low PTH, mostly seen as a consequence of neck surgery. Current management is the prescription of calcium and vitamin D, but the definitive treatment is parathyroid allotransplantation, which frequently triggers an immune response, thus cannot achieve the expected success. To overcome this problem, encapsulation of allogeneic cells is the most promising method. By optimizing the standard alginate cell encapsulation technique with parathyroid cells under high-voltage application, the authors reduced the size of parathyroid-encapsulated beads and evaluated these samples in vitro and in vivo
.
Methods
Parathyroid cells were isolated, and standard-sized alginate macrobeads were prepared without any electrical field application, while microbeads in smaller sizes (< 500 µm), by the application of 13 kV. Bead morphologies, cell viability, and PTH secretion were evaluated in vitro for four weeks. For the in vivo part, beads were transplanted into Sprague–Dawley rats, and after retrieval, immunohistochemistry and PTH release were evaluated in addition to the assessment of cytokine/chemokine levels.
Results
The viability of parathyroid cells in micro- and macrobeads did not differ significantly. However, the amount of in vitro PTH secretion from microencapsulated cells was significantly lower than that from macroencapsulated cells, although it increased throughout the incubation period. Immunohistochemistry of PTH staining in both of the encapsulated cells identified as positive after retrieval.
Conclusion
Contrary to the literature, a minimal in vivo immune response was developed for alginate-encapsulated parathyroid cells, regardless of bead size. Our findings suggest that injectable, micro-sized beads obtained using high-voltage may be a promising method for a non-surgical transplantation approach.
Graphical abstract</description><subject>Alginic acid</subject><subject>Cell size</subject><subject>Cell viability</subject><subject>Chemokines</subject><subject>Encapsulation</subject><subject>Endocrinology</subject><subject>Hypoparathyroidism</subject><subject>Immune response</subject><subject>Immunohistochemistry</subject><subject>Internal Medicine</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Metabolic Diseases</subject><subject>Microencapsulation</subject><subject>Microspheres</subject><subject>Original Article</subject><subject>Parathyroid hormone</subject><subject>Transplantation</subject><subject>Vitamin D</subject><subject>Voltage</subject><issn>1720-8386</issn><issn>0391-4097</issn><issn>1720-8386</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp9kUtLxTAQhYMoXl9_wIUE3LipJp2myV3KxRdccaPrME1TzaU3rUkr-O-N1hcuXCQZyHfODHMIOeTslDMmz2LBSq4ylkM6TIpMbpAdLnOWKVDl5q96RnZjXDEGEpTcJjMo57IsVLFD6ltnQme9wT6OLQ6u87RraI8Bh6fX0LmaGtu2kb44pLa1ZgjO0MbZtqboa-o7n8UxPDqDLR0C-ti36IfJCPs-dGie9slWg220B5_vHnm4vLhfXGfLu6ubxfkyMyDFkEkQ1lhVgqzyRiDHhkM1VxXYHOo5VMKIUvGiKECgUjlv-LySlamTKF05hz1yMvmmts-jjYNeu_g-PnrbjVHnSiQxSFkm9PgPuurG4NN0iZIgVSEKlqh8otKOYgy20X1wawyvmjP9noGeMtApA_2RgZZJdPRpPVZrW39LvpaeAJiAmL78ow0_vf-xfQN7uZNh</recordid><startdate>20231101</startdate><enddate>20231101</enddate><creator>Karabıyık Acar, Ö.</creator><creator>Başoğlu, H.</creator><creator>Keğin, M.</creator><creator>Nozhatzadeh, G. D.</creator><creator>Hacıhasanoğlu, E.</creator><creator>Tuncer, A. A.</creator><creator>Şahin, F.</creator><creator>Torun Köse, G.</creator><creator>Aysan, E.</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2697-6477</orcidid></search><sort><creationdate>20231101</creationdate><title>Microencapsulation of parathyroid cells via electric field and non-surgical transplantation approach</title><author>Karabıyık Acar, Ö. ; Başoğlu, H. ; Keğin, M. ; Nozhatzadeh, G. D. ; Hacıhasanoğlu, E. ; Tuncer, A. A. ; Şahin, F. ; Torun Köse, G. ; Aysan, E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-735ece8637b2f5a1af13b98b3e23d93b5c568144435a8821f19b7bcdececde213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Alginic acid</topic><topic>Cell size</topic><topic>Cell viability</topic><topic>Chemokines</topic><topic>Encapsulation</topic><topic>Endocrinology</topic><topic>Hypoparathyroidism</topic><topic>Immune response</topic><topic>Immunohistochemistry</topic><topic>Internal Medicine</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Metabolic Diseases</topic><topic>Microencapsulation</topic><topic>Microspheres</topic><topic>Original Article</topic><topic>Parathyroid hormone</topic><topic>Transplantation</topic><topic>Vitamin D</topic><topic>Voltage</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Karabıyık Acar, Ö.</creatorcontrib><creatorcontrib>Başoğlu, H.</creatorcontrib><creatorcontrib>Keğin, M.</creatorcontrib><creatorcontrib>Nozhatzadeh, G. D.</creatorcontrib><creatorcontrib>Hacıhasanoğlu, E.</creatorcontrib><creatorcontrib>Tuncer, A. A.</creatorcontrib><creatorcontrib>Şahin, F.</creatorcontrib><creatorcontrib>Torun Köse, G.</creatorcontrib><creatorcontrib>Aysan, E.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of endocrinological investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Karabıyık Acar, Ö.</au><au>Başoğlu, H.</au><au>Keğin, M.</au><au>Nozhatzadeh, G. D.</au><au>Hacıhasanoğlu, E.</au><au>Tuncer, A. A.</au><au>Şahin, F.</au><au>Torun Köse, G.</au><au>Aysan, E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microencapsulation of parathyroid cells via electric field and non-surgical transplantation approach</atitle><jtitle>Journal of endocrinological investigation</jtitle><stitle>J Endocrinol Invest</stitle><addtitle>J Endocrinol Invest</addtitle><date>2023-11-01</date><risdate>2023</risdate><volume>46</volume><issue>11</issue><spage>2257</spage><epage>2267</epage><pages>2257-2267</pages><issn>1720-8386</issn><issn>0391-4097</issn><eissn>1720-8386</eissn><abstract>Purpose
Hypoparathyroidism is a rare disease with low PTH, mostly seen as a consequence of neck surgery. Current management is the prescription of calcium and vitamin D, but the definitive treatment is parathyroid allotransplantation, which frequently triggers an immune response, thus cannot achieve the expected success. To overcome this problem, encapsulation of allogeneic cells is the most promising method. By optimizing the standard alginate cell encapsulation technique with parathyroid cells under high-voltage application, the authors reduced the size of parathyroid-encapsulated beads and evaluated these samples in vitro and in vivo
.
Methods
Parathyroid cells were isolated, and standard-sized alginate macrobeads were prepared without any electrical field application, while microbeads in smaller sizes (< 500 µm), by the application of 13 kV. Bead morphologies, cell viability, and PTH secretion were evaluated in vitro for four weeks. For the in vivo part, beads were transplanted into Sprague–Dawley rats, and after retrieval, immunohistochemistry and PTH release were evaluated in addition to the assessment of cytokine/chemokine levels.
Results
The viability of parathyroid cells in micro- and macrobeads did not differ significantly. However, the amount of in vitro PTH secretion from microencapsulated cells was significantly lower than that from macroencapsulated cells, although it increased throughout the incubation period. Immunohistochemistry of PTH staining in both of the encapsulated cells identified as positive after retrieval.
Conclusion
Contrary to the literature, a minimal in vivo immune response was developed for alginate-encapsulated parathyroid cells, regardless of bead size. Our findings suggest that injectable, micro-sized beads obtained using high-voltage may be a promising method for a non-surgical transplantation approach.
Graphical abstract</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>36976484</pmid><doi>10.1007/s40618-023-02075-7</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-2697-6477</orcidid></addata></record> |
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| subjects | Alginic acid Cell size Cell viability Chemokines Encapsulation Endocrinology Hypoparathyroidism Immune response Immunohistochemistry Internal Medicine Medicine Medicine & Public Health Metabolic Diseases Microencapsulation Microspheres Original Article Parathyroid hormone Transplantation Vitamin D Voltage |
| title | Microencapsulation of parathyroid cells via electric field and non-surgical transplantation approach |
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