Proteomic identification of metabolic changes in Paracoccidioides brasiliensis induced by a nitroheteroarylchalcone

To access the metabolic changes caused by a chalcone derivative ( ) through a proteomic approach. Proteomic analysis was performed after 9 h of yeast ( ) cell incubation with the at MIC. The proteomic findings were validated through and assays. Exposure to the compound led to the downregulation of proteins associated with glycolysis and gluconeogenesis, β-oxidation, the citrate cycle and the electron transport chain. caused an energetic imbalance in the fungus metabolism and deep oxidative stress. Additionally, the molecular docking approach pointed to this molecule as a putative competitive inhibitor of DHPS.