Ultrasonic–microwave synergistic supramolecular solvent liquid–liquid microextraction of trace biogenic amines in fish and beer based on solidification of floating organic droplet

[Display omitted] •·A novel UMS-SUPRAS-SFO-LLME method for BAs extraction is reported.•·The prepared SUPRAS has a low viscosity, density, and melting point.•·The ultrasonic–microwave synergistic treatment improved the extraction effect.•·The dynamics and thermodynamics of the UMS-SUPRAS-SFO-LLME process were explored.•·This extraction method was coupled to HPLC with low LODs and high recoveries. It is important to detect the presence of biogenic amines (BAs) as indicators of food freshness. The purpose of this study was to develop a novel ultrasonic–microwave synergistic supramolecular solvent liquid–liquid microextraction based on solidification of floating organic droplet (UMS-SUPRAS-SFO-LLME) combined with high-performance liquid chromatography for the determination of BAs. The physical properties and microstructure of SUPRAS based on 1-dodecanol and tetrahydrofuran were studied, and the extraction conditions such as the SUPRAS volume, the UMS process, and the centrifugal conditions were optimized. The results for the extraction kinetics and thermodynamics showed that UMS-SUPRAS-SFO-LLME is a spontaneous, endothermic diffusion process. The linear ranges of this method are 0.1–2.0 × 105 ng·mL−1 (R2 > 0.994), the limits of detection are 4.0 × 10-3-6.0 × 10-2 ng·mL−1, and the recoveries were 96.28–103.15%. Compared with existing analysis methods, UMS-SUPRAS-SFO-LLME is a sensitive, green and economical sample pretreatment method for analyzing the enrichment of BAs in beer and fish.