A hyperthermophilic anaerobic fermentation platform for highly efficient short chain fatty acids production from thermal hydrolyzed sludge

•A carboxylate platform of HAF for SCFAs production from THS was established.•High production of SCFAs with high acetate ratio were achieved in the HAF.•The HAF had the highest acetate production rate under the optimum SRT of 3 d.•Nearly no biogas produced from the HAF due to inhibited methanogenesis.•Multiple metabolic pathways in the HAF were enhanced under the optimum SRT. In this study, a carboxylate platform of hyperthermophilic (70 ℃) anaerobic fermentation (HAF) for short chain fatty acids (SCFAs) production from thermal hydrolyzed sludge (THS) was established. The long-term performance for SCFAs production and the microbial communities of this HAF under different SRTs were systematically investigated. Under the optimum SRT of 3 d, the HAF had the highest acetate production rate of 1.12 g COD/L/d which accounted for 60% in SCFAs. It also rendered a good performance in SCFAs production, with concentration, production rate and yield of 6.61 g COD/L, 1.86 g COD/L/d and 324 g COD/kg VSSin, respectively. Nearly no biogas produced from this system, which reduced the loss of carbon sources from the system. This was due to the inhibition of methanogenesis by the hyperthermophilic condition and the high content of total ammonia nitrogen (TAN) and free ammonia nitrogen (FAN). Tepidimicrobium, Bhargavaea and XBB1006 were the dominant genus-level biomarkers under the optimum SRT, which facilitated the decomposition of monosaccharides, amino acids, terpenoids and polyketides into SCFAs. This work provides an applicable anaerobic carboxylate platform for highly efficient SCFAs production from excess sludge. [Display omitted]