PredDRBP-MLP: Prediction of DNA-binding proteins and RNA-binding proteins by multilayer perceptron

Proteins interact with many molecules in order to maintain the vital activities in cells. Proteins that interact with DNA are called DNA-binding proteins (DBP), and proteins that interact with RNA are called RNA-binding proteins (RBP). Since DBPs and RBPs are involved in critical biological processes, their classification is quite important. Although the convolutional neural network and bidirectional long-short-term memory hybrid model (CNN-BiLSTM) is very popular in DBP and RBP classification, it has problems such as requirement of high processing power and long training time. Therefore, a multilayer perceptron (MLP) based predictor, PredDRBP-MLP (Predictor of DNA-Binding Proteins and RNA-Binding Proteins - Multilayer Perceptron) was developed in this study. PredDRBP-MLP is an artificial learning model that performs multi-class classification of DBPs, RBPs and non-nucleic acid-binding proteins (NNABP). PredDRBP-MLP achieved quite successful results on the independent dataset, specifically in the NNABP class, compared to the existing predictors, in addition to requiring lower processing power and being able to train quicker compared to CNN-BiLSTM based predictors. In NNABP class, PredDRBP-MLP predictor achieved 0.578 precision, 0.522 recall and 0.549 F1-score, while other multi-class predictor achieved 0.486 precision, 0.183 recall and 0.266 F1-score. A desktop application was developed for PredDRBP-MLP. The application is freely accessible at https://sourceforge.net/projects/preddrbp-mlp. [Display omitted] •A multilayer perceptron-based model was created for multiclass classification of DNA-binding and RNA-binding proteins.•Gaussian noise was included to the model to solve the cross-prediction problem and generalize the predictor.•Compared to the existing predictors, higher performance metrics were obtained in the non-nucleic acid-binding proteins.•A user-friendly desktop application was developed from the predictor created in this study.