Brachybacterium atlanticum sp. nov., a novel marine bacterium isolated from the Atlantic Ocean

A bacterial strain, PhyBa_CO2_2 , was isolated from the North Atlantic Gyre, offshore Terceira Island in the Azores. Initially, the NCBI nucleotide blast analysis based on 16S rRNA gene sequences revealed that the strain belongs to the genus , with a 100 % identity with LMG 19861 . However, further genomic characterization through average nucleotide identity (ANI) and digital DNA-DNA hybridization analyses showed values of 96.06 and 64.80 %, respectively. Comparative genomics also highlighted differences in gene content. The genome size of PhyBa_CO2_2 is 3.6 Mbp and the DNA G+C content is 72.1 mol%. Chemotaxonomic analysis demonstrated that the composition of the fatty acids was mainly composed of anteiso-C (46.04 %), iso-C (13.70 %) and anteiso-C (9.48 %), and the polar lipids were mainly diphosphatidylglycerol, phosphatidylglycerol and two unidentified glycolipids. Furthermore, the diagnostic amino acid of the cell wall was -diaminopimelic acid and the predominant menaquinone was MK7. Finally, phenotypic analysis revealed differences in biochemical profiles between PhyBa_CO2_2 and its closely related strains in terms of indole production, urease and β-glucuronidase activity. Therefore, based on the genomic, chemotaxonomic and phenotypic data obtained, we concluded that strain PhyBa_CO2_2 represents a new species, for which the name sp. nov. is proposed in reference to its isolation site. The type strain is PhyBa_CO2_2 (=DSM 114113 = CECT 30695 ).