A synergistic approach combining Adsorption and Biodegradation for effective treatment of Acid Blue 113 dye by Klebsiella grimontii entrapped Graphene Oxide-Calcium Alginate Hydrogel Beads

[Display omitted] •Klebsiella grimontii entrapped Graphene Oxide-Calcium Alginate Beads had developed.•KG-GO-CA effectively used for AB 113 removal up to 12th regeneration cycle in FBBR.•Bacterial toxicity assessment ensured the safe disposal of AB 113 treated water.•Langmuir isotherm was best fitted with Qm of 203.83 mg/g and Ki of 0.0101 L/g. This study investigated the degradation of Acid Blue 113 (AB 113) dye using Klebsiella grimontii entrapped Graphene Oxide-Calcium Alginate Hydrogel beads (KG-GO-CA) in a Fluidized Bed Bioreactor (FBBR) under varying inlet loading rates. The minimum fluidization velocity of the KG-GO-CA hydrogel beads in FBBR was found to be 0.15 mm/s. The KG-GO-CA beads showed a maximum removal efficiency of 94.6% at an inlet flow rate of 20 mL/h over 15 days. Reusability studies indicated a removal efficiency of 70.6 ± 2.5% for AB 113 after the 12th cycle. Langmuir adsorption isotherm showed the best fit (R2 = 0.98724) with model parameters of Qm (203.83 mg/g) and Ki (0.0101 L/g). The study also confirmed that treated wastewater was more environmentally safe for domestic and commercial uses than untreated wastewater. The research highlights the potential use of KG-GO-CA hydrogel beads for removing dyes from wastewater.