Polyvinyl alcohol electrospun nanofiber membrane based solid-phase extraction for monitoring administered aminoglycoside antibiotics in various animal-derived foods

[Display omitted] •A method for monitoring administered AGs in various animal-derived foods is developed.•PVA-NFsM achieves effective extraction of targets as a new SPE adsorbent.•PVA NFsM shows good purification ability for sample matrix and reusability.•The accuracy and sensitivity of the method are equivalent to or better than existing methods.•The good practicality of the developed method was verified by testing actual samples. This work aims to develop a widely applicable method to monitor administered AGs in various animal-derived food samples to ensure food safety. A polyvinyl alcohol electrospun nanofiber membrane (PVA NFsM) was synthesized and employed as solid-phase extraction (SPE) sorbent, in combination with UPLC-MS/MS, for the simultaneous detection of ten AGs in nine types of animal-derived food samples. PVA NFsM exhibited excellent adsorption performance for the targets (with an adsorption rate of over 91.09%), good matrix purification ability (with a reduction of 7.65%-77.47% in matrix effect after SPE), and good recyclability (can be reused 8 times). The method displayed a linear range of 0.1–25000 μg/kg and attained limits of detection for AGs were 0.03–15 μg/kg. Spiked samples demonstrated a recovery of 91.72%-100.04% with a precision of