Raman spectroscopy for postmortem interval estimation of human skeletal remains: A scoping review

Estimating postmortem intervals (PMI) is crucial in forensic investigations, providing insights into criminal cases and determining the time of death. PMI estimation relies on expert experience and a combination of thanatological data and environmental factors but is prone to errors. The lack of reliable methods for assessing PMI in bones and soft tissues necessitates a better understanding of bone decomposition. Several research groups have shown promise in PMI estimation in skeletal remains but lack valid data for forensic cases. Current methods are costly, time‐consuming, and unreliable for PMIs over 5 years. Raman spectroscopy (RS) can potentially estimate PMI by studying chemical modifications in bones and teeth correlated with burial time. This review summarizes RS applications, highlighting its potential as an innovative, nondestructive, and fast technique for PMI estimation in forensic medicine. Determining the postmortem interval (PMI) in forensic medicine is crucial for criminal investigations but challenging due to environmental factors affecting decomposition. Raman spectroscopy (RS) is a fast and objective method with valuable applications in estimating PMIs for forensic and anthropological analyses. This review emphasizes RS's significance in understanding bone decomposition and its contribution to analytical chemistry.