Detrimental consequences of tebuconazole on redox homeostasis and fatty acid profile of honeybee brain

Excessive use of azole fungicides in agriculture poses a potential threat to honeybees and other pollinator insects; however, the detailed effects of these molecules remain largely unclear. Hence, in the present study it was aimed to investigate the acute sublethal effects of tebuconazole on the redox homeostasis and fatty acid composition in the brain of honeybees. Our findings demonstrate that tebuconazole decreased total antioxidant capacity, the ratio of reduced to oxidized glutathione and disturbed the function of key antioxidant defense enzymes along with the induction of lipid peroxidation indicated by increased malondialdehyde levels, while it also altered the fatty acid profile of the brain. The present study highlights the negative impact of tebuconazole on honeybees and contributes to the understanding of potential consequences related to azole exposure on pollinator insects’ health, such as the occurrence of colony collapse disorder. [Display omitted] •Tebuconazole leads to detrimental effects on redox homeostasis of honeybee brain.•Glutathione system and redox enzymes were severely affected.•Increased malondialdehyde concentration refers to intense lipid peroxidation.•Fatty acid profile was intensely altered following tebuconazole treatment.