Structural characterization and anti-oxidation activity of pectic polysaccharides from Swertia mileensis
In this study, we isolated the pectic polysaccharide WSMP-A2b (37 kDa) from the stems and leaves of Swertia mileensis, and we investigated its compositional/structural features and antioxidant activity. FT-IR, NMR, monosaccharide composition, enzymatic hydrolysis and methylation analyses indicated t...
Gespeichert in:
Veröffentlicht in: | International journal of biological macromolecules 2023-09, Vol.248, p.125896-125896, Article 125896 |
---|---|
Hauptverfasser: | , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | In this study, we isolated the pectic polysaccharide WSMP-A2b (37 kDa) from the stems and leaves of Swertia mileensis, and we investigated its compositional/structural features and antioxidant activity. FT-IR, NMR, monosaccharide composition, enzymatic hydrolysis and methylation analyses indicated that WSMP-A2b is composed of rhamnogalacturonan I (RG-I), rhamnogalacturonan II (RG-II) and homogalacturonan (HG) domains with mass ratios of 2.1:1.0:2.2. The RG-I domain is primarily substituted with α-L-1,5-arabinan and type II arabinogalactan (AG-II) side chains, as well as minor contributions of β-D-1,4-galactan and/or type I arabinogalactan (AG-I) side chains. The HG domain was released in the form of un-esterified and partly methyl-esterified and/or acetyl-esterified oligogalacturonides with a 1 to 7 degree of polymerization after endo-polygalacturonase degradation. WSMP-A2b showed stronger antioxidant activity in vitro, in part this might due to the presence of galacturonic acid (GalA). In addition, WSMP-A2b exerted a protective effect on tert-butyl hydroperoxide (tBHP)-induced oxidative stress in INS-1 cells by reducing reactive oxygen species (ROS) production and increasing the glutathione/oxidized glutathione (GSH/GSSG) ratio. Our results provide crucial structural information on this pectic polysaccharide from Swertia mileensis, thus prompting further investigation into its structure-activity relationship. |
---|---|
ISSN: | 0141-8130 1879-0003 |
DOI: | 10.1016/j.ijbiomac.2023.125896 |