Light stress induced DMS(P) production in Skeletonema costatum: An experimental approach and field observation

Dimethylsulphide is a dominant biogenic sulphur anti-greenhouse gas produced by marine phytoplankton. A non-axenic culture of Skeletonema costatum was studied to comprehend the effects of different growth stages and light stress on DMSP/DMS production. The intracellular DMSP concentration increased during late exponential to mid-stationary phase and attained a maximum (0.59 pg S cell−1) during the stationary phase, indicating more contribution from actively dividing smaller cells. Likewise, exposure to first light after a 12-hour dark phase caused stress, invariably leading to elevated levels of DMS (~9 fold). These observations were upheld by additional laboratory and field experiments, and a field time-series observation, which recorded higher DMS concentrations during exposure to first light after a dark cycle and during early mornings, respectively. While our study depicts the variable DMSP and DMS concentrations during different growth stages of S. costatum, it gives new information on the effect of light stress on DMS production. •Maximum DMSP production occurred during late exponential to mid-stationary phase.•Actively dividing smaller cells with a higher surface-to-volume ratio produce DMSP.•First light exposure induces DMS production in Skeletonema costatum.•Laboratory experiments and field time series indicate a similar result.•More research is needed to understand the implications on DMS flux and budget.