Role of kisspeptin-10 and betacellulin in control of feline ovarian cell functions

The action of betacellulin (BTC) on basic ovarian cell functions and interrelationships with kisspeptin (KISS) was investigated. For this purpose, we examined (1) the effect of the addition of BTC (0, 1, 10, and 100 ng/ml) given alone or in combination with KISS (10 ng/ml) on cultured feline ovarian fragments or granulosa cells. Viability, proliferation (accumulation of cyclin B1) and apoptosis (accumulation of bax), and the release of steroid hormones (progesterone, testosterone, and estradiol) were analyzed by using the Trypan blue exclusion test, quantitative immunocytochemistry, and ELISA. The addition of KISS alone increased proliferation, apoptosis, progesterone, estradiol release, and decreased testosterone but did not affect viability. The addition of BTC alone decreased cell proliferation, apoptosis, progesterone, testosterone, and estradiol release but did not influence viability. Furthermore, BTC mainly inhibited the stimulatory action of KISS on feline ovarian functions. The findings of our study suggest the effects of KISS on basic ovarian functions. We also observed the influence of BTC on these functions and its ability to modify the effects of KISS on these processes.