Polysaccharide of Atractylodes macrocephala Koidz(PAMK) protects against DEHP-induced apoptosis in grass carp hepatocytes

Di(2-ethylhexyl) phthalate (DEHP) is a new environmental pollutant, which is widely used in plastic additives. DEHP and its metabolites pollute surface water and threaten the survival of fish. In order to investigate the mechanism of DEHP-induced apoptosis on grass carp hepatocytes, we treated grass carp hepatocytes with DEHP, and selected Atractylodes macrocephala Koidz (PAMK) to study its inhibitory effect on DEHP. The results showed that after DEHP exposure, apoptosis related proteins expression were increased significantly, leading to hepatocytes apoptosis. Moreover, AO/EB staining and Hoechst staining also showed that the number of apoptotic cells increased after DEHP exposure. It should be noted that PAMK simultaneous treatment could alleviate apoptosis induced by DEHP. The innovation of this study is that the application of Chinese herbal medicine (PAMK) to antagonize the damage of DEHP in fish was investigated for the first time. This study indicated that traditional Chinese medicine can also be used in fish production to reduce the accumulation of food-derived drugs. •Polysaccharide of Atractylodes macrocephala Koidz (PAMK) can alleviate DEHP-induced apoptosis in grass carp hepatocytes.•PAMK relieves the toxicity of DEHP.•Traditional Chinese medicine could be applied fish farming in the future.