Investigation for the presence of bacteria and antimicrobial resistance genes in sea snails ( Rapana venosa )

The aims of this study were to search for the presence of bacteria in sea snails ( ) by using culturomics and Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and the antibiotic resistance/susceptibility of the sea snails. The anti-microbial susceptibilities of Gram-negative bacteriawas assessed by the Kirby-Bauer disk diffusion method, the presence of the genes (mcr-1 to -5), the major carbapenemase and β-lactamase resistant genes in Gram-negative bacteria, using mPCR method and 16S rRNA sequence analysis of isolates. Bacterial growth accounted for 100% and 94.2% in the samples of intestine and meat, respectively, in the snails. The main organisms identified by MALDI-TOF MS were subsp. salmonicida at 33.7%, followed by at 9.6% (10/104) and at 7.7% in meat and intestine samples. and are intrinsic or chromosomally-mediated resistant against ampicillin. No genes ( -1 to -5), the major carbapenemase and β-lactamase resistant genes were found. subsp. showed very low levofloxacin and meropenem resistance levels at 2.9%. When the sequence was searched in the Blast database, the genome of isolate showed high similarity with the sequences. Conclusions. The findings obtained not only provide data about the proportion of bacteria in the gut and meat of the sea snails and their antibiotic resistance/susceptibility, but also show the absence of carbapenemase, colistin, and β-lactamase resistant genes among bacterial isolates from sea snail gut microbes.