Alkalihalobacillus deserti sp. nov., Isolated from the Saline–Alkaline Soil
A bacterial strain, designated TRPH29 T , was isolated from saline-alkaline soil, collected from the southern edge of the Gurbantunggut desert, Xinjiang, People’s Republic of China. The isolate was Gram-staining positive, facultatively anaerobic, straight rods. Growth occurred at 15–40 °C (optimum,...
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Veröffentlicht in: | Current microbiology 2023-08, Vol.80 (8), p.261-261, Article 261 |
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Zusammenfassung: | A bacterial strain, designated TRPH29
T
, was isolated from saline-alkaline soil, collected from the southern edge of the Gurbantunggut desert, Xinjiang, People’s Republic of China. The isolate was Gram-staining positive, facultatively anaerobic, straight rods. Growth occurred at 15–40 °C (optimum, 28 °C), pH 8.0–13.0 (optimum, 10.0), and in the presence of 0–15% (w/v) NaCl (optimum, 2%). Phylogenetic analysis using 16S rRNA gene sequence indicated that strain TRPH29
T
showed the highest sequence similarities to
Alkalihalobacillus krulwichiae
(98.31%)
, Alkalihalobacillus wakoensis
(98.04%)
,
and
Alkalihalobacillus akibai
(97.69%). Average nucleotide identity (ANI) and digital DNA-DNA hybridization values between strain TRPH29
T
and
Alkalihalobacillus krulwichiae
,
Alkalihalobacillus wakoensis
,
Alkalihalobacillus akibai
were in the range of 73.62–75.52% and 15.0–21.20%, respectively. Results of genome analyses indicated that the genome size of strain TRPH29
T
was 5.05 Mb, with a genomic DNA G + C content of 37.30%. Analysis of the cellular component of strain TRPH29
T
revealed that the primary fatty acids were anteiso-C
15:0
and iso-C
15:0
, and the polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified glycolipid, and an unidentified phospholipid. The predominant respiratory quinone was MK-7. Based on the genomic, phylogenetic, phenotypic and chemotaxonomic analyses, strain TRPH29
T
represents a novel species of the genus
Alkalihalobacillus
, for which the name
Alkalihalobacillus deserti
sp. nov. is proposed. The type strain is TRPH29
T
(= CGMCC 1.19067
T
= NBRC 115475
T
). |
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ISSN: | 0343-8651 1432-0991 |
DOI: | 10.1007/s00284-023-03353-6 |