Alkalihalobacillus deserti sp. nov., Isolated from the Saline–Alkaline Soil

A bacterial strain, designated TRPH29 T , was isolated from saline-alkaline soil, collected from the southern edge of the Gurbantunggut desert, Xinjiang, People’s Republic of China. The isolate was Gram-staining positive, facultatively anaerobic, straight rods. Growth occurred at 15–40 °C (optimum,...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Current microbiology 2023-08, Vol.80 (8), p.261-261, Article 261
Hauptverfasser: Rouzi, Zulihumaer, Li, Yu-Qian, Aosiman, Muyesaier, Abudourousuli, Dilireba, Liu, Lan, Alkhalifah, Dalal Hussien M., Hozzein, Wael N., An, Deng-Di, Li, Wen-Jun
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:A bacterial strain, designated TRPH29 T , was isolated from saline-alkaline soil, collected from the southern edge of the Gurbantunggut desert, Xinjiang, People’s Republic of China. The isolate was Gram-staining positive, facultatively anaerobic, straight rods. Growth occurred at 15–40 °C (optimum, 28 °C), pH 8.0–13.0 (optimum, 10.0), and in the presence of 0–15% (w/v) NaCl (optimum, 2%). Phylogenetic analysis using 16S rRNA gene sequence indicated that strain TRPH29 T showed the highest sequence similarities to Alkalihalobacillus krulwichiae (98.31%) , Alkalihalobacillus wakoensis (98.04%) , and Alkalihalobacillus akibai (97.69%). Average nucleotide identity (ANI) and digital DNA-DNA hybridization values between strain TRPH29 T and Alkalihalobacillus krulwichiae , Alkalihalobacillus wakoensis , Alkalihalobacillus akibai were in the range of 73.62–75.52% and 15.0–21.20%, respectively. Results of genome analyses indicated that the genome size of strain TRPH29 T was 5.05 Mb, with a genomic DNA G + C content of 37.30%. Analysis of the cellular component of strain TRPH29 T revealed that the primary fatty acids were anteiso-C 15:0 and iso-C 15:0 , and the polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified glycolipid, and an unidentified phospholipid. The predominant respiratory quinone was MK-7. Based on the genomic, phylogenetic, phenotypic and chemotaxonomic analyses, strain TRPH29 T represents a novel species of the genus Alkalihalobacillus , for which the name Alkalihalobacillus deserti sp. nov. is proposed. The type strain is TRPH29 T (= CGMCC 1.19067 T  = NBRC 115475 T ).
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-023-03353-6