Spatiotemporal dynamics of focal adhesion kinase
We designed FAK-SPARK, a fluorescent reporter of focal adhesion kinase (FAK) activity based on phase separation. FAK-SPARK revealed polarized FAK activity within single focal adhesions in the leading edges of migrating cells. By combining FAK-SPARK with DNA tension probes, we showed that FAK activit...
Gespeichert in:
Veröffentlicht in: | Nature chemical biology 2023-12, Vol.19 (12), p.1444-1445 |
---|---|
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | We designed FAK-SPARK, a fluorescent reporter of focal adhesion kinase (FAK) activity based on phase separation. FAK-SPARK revealed polarized FAK activity within single focal adhesions in the leading edges of migrating cells. By combining FAK-SPARK with DNA tension probes, we showed that FAK activity is proportional to the tension strength. |
---|---|
ISSN: | 1552-4450 1552-4469 |
DOI: | 10.1038/s41589-023-01371-w |