Co-exposure effects of lead and TiO2 nanoparticles in primary kidney cell culture from the freshwater fish Hoplias malabaricus

This study evaluated the effects of Lead (Pb) and titanium dioxide nanoparticles (TiO2 NPs) alone or in combination in anterior kidney macrophages of the freshwater fish Hoplias malabaricus, naïve or stimulated with 1 ng.mL-1 lipopolysaccharide (LPS). Pb (1 ×10-5 to 1 ×10-1 mg.mL-1) or TiO2 NPs (1.5 ×10-6 to 1.5 ×10-2 mg.mL-1) reduced cell viability despite LPS stimulation, especially Pb 10-1 mg.mL-1. In combination, lower concentrations of NPs intensified Pb-induced cell viability reduction while higher concentrations restored the cell viability independently of LPS stimulation. Basal and LPS- induced NO production was reduced by both TiO2 NPs and Pb isolated. The combination of both xenobiotics avoided this reduction of NO production by the isolated compounds at lower concentrations but the protective effect was lost as the concentrations increased. None xenobiotic increase DNA fragmentation. Therefore, at specific conditions, TiO2 NPs may have a protective effect over Pb toxicity, may also provide additional toxicity at higher concentrations. •The effects of Pb and TiO2 nanoparticles were evaluate fish kidney macrophages.•Pb reduced the cell viability while TiO2 nanoparticles showed little effect.•The co-exposure showed a protective effect of the nanoparticles on effects of Pb in cell viability.•The protective effect did not occurred for NO production.•The effects on these concentrations cannot be attribute to DNA damage.