Microextraction by packed sorbent of some β-blocker drugs with chitosan@mof-199 bio-composite in human saliva, plasma, and urine samples

The current study introduces microextraction by packed sorbent (MEPS) to extract three beta-blocker drugs (propranolol, atenolol, and betaxolol) from biological samples. The separation and detection of the drugs were performed by high performance liquid chromatography followed by UV detection. A green approach was applied for synthesizing chitosan@MOF-199 bio-composite, which was packed into the initial part of a metal spinal (22 gage). The effective parameters on the adsorption and desorption efficiencies, including sample solution pH, eluent flow rate, cycle numbers, type and volume of eluent solvent were evaluated and optimized. Under optimal conditions linear ranges (LRs = 5–600 µg L−1), limits of detection (LODs = 1.5–4.5 µg L−1), and relative standard deviations (RSDs% = 4.7 −5.3% with three replicates and concentration of 100 µg L−1) were obtained. Relative recoveries (RR%) for plasma (77–99%), saliva (81–108%), and urine (80–112%) samples were obtained. In this study, the drug release profile of propranolol in urine was evaluated. The results showed that the highest amount of propranolol is released 4 h after taking the drug. Based on the obtained results, this is an effective, fast, sensitive, reproducible, green, and user-friendly method for beta-blocker drug extraction in biological samples. [Display omitted] •A green bio-composite based chitosan and nanocrystals of MOF-199 was synthesized.•The CS@MOF-199 bio-composite was characterized using various techniques.•The CS@MOF-199 was used in MEPS for extraction of some beta-blocker drugs.•The method has high sensitivity with a good linearity for atenolol, propranolol, and betaxolol.•Acceptable RR% and ME% were obtained for measuring β-blockers in biological samples.