Fine mapping of genes controlling pigment accumulation in oilseed rape (Brassica napus L.)

Purple/red appearance is one of the common phenotypic variations in leaves, stems, and siliques of oilseed rape ( Brassica napus L.) but very rare in flowers. In this study, the causal genes for the purple/red traits in stems and flowers in two accessions of oilseed rape (DH_PR and DH_GC001, respectively) derived from the wide hybridization were fine mapped, and candidate genes were determined by methods combined with bulked segregant analysis (BSA) and RNA-seq analysis. Both traits of purple stem and red flowers were mapped to the locus as AtPAP2 homologous genes ( BnaPAP2.C6a and BnaPAP2.A7b , respectively) belonging to the R2R3-MYB family . Sequence comparisons of full-length allelic genes revealed several InDels and SNPs in intron 1 as well as exons, and completely different promoter region of BnaPAP2.C6a and a 211 bp insertion was identified in the promoter region of BnaPAP2.A7b of DH_GC001. Our results not only contribute to a better understanding of anthocyanin inheritance in B. napus , but also provide a useful toolbox for future breeding of cultivars with purple/red traits through the combination of different functional alleles and homologs.