Potential of biochar for hydrocarbon degradation of crude oil–contaminated soils

A greenhouse experiment was conducted to assess the effect of phytoremediation and biochar application on hydrocarbon degradation in crude oil–contaminated soils. The experiment consisted of four levels of biochar application (0, 5, 10, and 15 t/ha) and the presence or absence of Vigna unguiculata (cowpea; +C, −C) replicated thrice and arranged in a 4 × 2 × 3 factorial completely randomized design. Samples were taken on days 0, 30, and 60 for total petroleum hydrocarbon (TPH) analysis. A significantly higher TPH degradation efficiency of 69.2% (7033 mg/kg) was observed in contaminated soils amended with 15 t/ha biochar only after 60 days of incubation. Highly significant interactions were observed between biochar × plant (p