Remineralization Potential of a Novel Biomimetic Material (Self-assembling Peptide P 11-4 ) on Early Enamel Caries: An In Vitro Study

To assess the remineralizing potential of self-assembling peptide P and compare it to the remineralizing potential of fluoride varnish using DIAGNOdent , as well as the amount of mineral gain after application of fluoride varnish and self-assembling peptide P . This study included 20 premolars extracted during orthodontic therapy with all surfaces intact and free of hypoplastic regions, white spot lesions (WSL) and dental caries. The teeth sample for Curodont Repair (self-assembling P ) and Bifluorid 10® (fluoride varnish) was equally divided. On each tooth surface, a 2 × 2 mm window was created. The samples were immersed in a demineralizing solution for 96 hours before being subjected to DIAGNOdent pen reading, ICDAS-II scoring, and scanning electron microscopy-energy-dispersive X-ray (SEM-EDX) analysis on one half of the sample. The remineralizing agents were applied to the second half of the sample according to the manufacturer's instructions and placed in artificial saliva for 21 days, with the artificial salvia being replaced every 24 hours. After 21 days, the second half of the sample was subjected to DIAGNOdent pen reading, ICDAS-II score, and SEM-EDX analysis. Following remineralization, the DIAGNOdent pen and ICDAS-II score values differed statistically between the two groups, with the Bifluorid 10® group reporting higher mean values ( > 0.05) using -test analysis. Energy-dispersive X-ray analysis using the -test revealed a statistically significant result for remineralization ( < 0.05), with Curodont Repair group (55.150.84) reporting better mean values than Bifluorid 10® for phosphorus and calcium, but Bifluorid 10® reporting a higher result in remineralization ( < 0.05) than Curodont Repair for fluoride. Curodont Repair showed better remineralizing potential compared with Bifluorid 10® varnish. In terms of the mineral gain, Curodont Repair showed better results for calcium and phosphorus post-remineralization. Whereas Bifluorid 10® showed a higher gain in terms of fluoride. Self-assembling peptide P can be used as an alternative to fluoride varnish for remineralizing WSL. Self-assembling polypeptide P is a novel remineralizing agent for initial enamel lesions, which is the least-invasive method of enamel remineralization.