Structure characterization and biological activities evaluation of two hetero-polysaccharides from Lepista nuda: Cell antioxidant, anticancer and immune-modulatory activities

Polysaccharides LNP-1 and LNP-2 were extracted and purified from Lepista nuda, and their structural characteristics and biological activities were evaluated. The molecular weights of LNP-1 and LNP-2 were determined to be 16,263 Da and 17,730 Da, respectively. The monosaccharide composition analysis showed that LNP-1 and LNP-2 were composed of fucose, mannose, glucose, and galactose in a molar ratio of 1.00:2.42:1.09:4.04 and 1.00:2.39:1.61:4.23, respectively. The structure analysis revealed that these two polysaccharides were mainly composed of T-Fuc, T-Man, T-Glc, 1,6-Glc 1,6-Gal, and 1,2,6-Man, 1,2,6-Gal. Additionally, LNP-2 contained an additional 1,4-Glc glycosidic linkage in comparison to LNP-1. Both LNP-1 and LNP-2 exhibited anti-proliferation effects on A375 cells, but not on HepG2 cells. Furthermore, LNP-2 showed better cellular antioxidant activity (CAA) than LNP-1. RT-PCR results indicated that LNP-1 and LNP-2 could induce macrophages to secrete immune-modulatory factors NO, IL-6, and TNF-α by regulating their mRNA expression. Overall, this study provides a theoretical basis for the further development of the structure-function relationship of polysaccharides from L. nuda.