An Ex Vivo Pilot Study to Assess the Feasibility of 3D Printing of Orbital Implants in Horses

•Since the development process and implantation of a custom-made, biocompatible, and economically accessible 3D-printed orbital implant has yet to be described in enucleated horses, the current study investigated its feasibility. A prototype was designed and implanted in twelve cadaver heads following a myoconjunctival enucleation.•Space for placement of a cosmetic ocular prosthesis, soft-tissue coverage of the implant, symmetry to the septum and horizontal symmetry were assessed.•The production of the implant and its adaptation and fixation within the orbit of the twelve cadaver heads was successful. Further studies need to be conducted to determine whether such prototype can be used in vivo. Severe ophthalmic conditions such as trauma, uveitis, corneal damage, or neoplasia can lead to eye removal surgery. Poor cosmetic appearance resulting from the sunken orbit ensues. The aim of this study was to demonstrate the feasibility of manufacturing a custom-made 3D-printed orbital implant made of biocompatible material for the enucleated horse and usable in conjunction to a corneoscleral shell. Blender, a 3D-image software, was used for prototype design. Twelve cadaver heads of adult Warmbloods were collected from the slaughterhouse. On each head, one eye was removed via a modified transconjunctival enucleation while the contralateral eye was kept intact as control. Ocular measurements were collected on each enucleated eye with the help of a caliper and used for prototype sizing. Twelve custom-made biocompatible porous prototypes were 3D-printed in BioMed Clear resin using the stereolithography technique. Each implant was fixated into the corresponding orbit, within the Tenon capsule and conjunctiva. Heads were frozen and thin slices were then cut in the transverse plane. A scoring system based on four criteria (space for ocular prosthesis, soft-tissue-coverage, symmetry to the septum, and horizontal symmetry), ranging from A (proper fixation) to C (poor fixation), was developed to evaluate implantation. The prototypes reached our expectations: 75% of the heads received an A score, and 25% a B score. Each implant cost approximately 7.30€ and took 5 hours for 3D-printing. The production of an economically accessible orbital implant made of biocompatible porous material was successful. Further studies will help determine if the present prototype is usable in vivo.