Parasite-host relations and new reports of helminths for Rhinella dorbignyi (Duméril & Bibron, 1841) (Anura: Bufonidae) from Neotropical region

This study aimed to analyze helminth assemblage in Rhinella dorbignyi regarding host gender, size and mass in two sampling sites in southern Brazil, and to report new parasite associations. Anurans (n = 100) were collected in two localities from Rio Grande do Sul (RS) state, Brazil, from 2017 to 2020. Nineteen taxa (adults and larval forms) belonging to Nematoda, Acanthocephala, Digenea, and Cestoda were found in different infection sites. Cosmocercidae gen. spp., Physaloptera liophis, Catadiscus sp., and Cylindrotaenia americana were the dominant taxa in helminth assemblage. Female anurans showed higher helminth species richness than males considering the total sample (two localities) or one of the two localities. However, prevalence and mean intensity of infection did not show any significant difference between genders. Mean intensity of infection was significantly higher (19.52) in Laranjal locality. Host body size does not influence helminth abundance, as infections did not show significant correlation snout-vent length (SVL) nor body mass (BM) of anurans. The findings reveal anurans of R. dorbignyi may be intermediate, paratenic and definitive host for these parasites. Plagiorchioidea helminths (Digenea), Physaloptera liophis, larvae of Acuariidae and Spiroxys sp. (Nematoda), and cystacanth of Lueheia sp. (Acanthocephala) constitute new records for R. dorbignyi. Additionally, this is the first record of Cylindrotaenia americana larvae in this host species. Resulting information increases the knowledge on biodiversity and parasite-host relations and may help future conservation programs developed in ecosystems in the extreme south of Brazil. [Display omitted] •Helminth richness recorded in Rhinella dorbignyi is composed of monoxenic and heteroxenic cycle taxa, thus this anuran can act as intermediate, paratenic and definitive hosts.•Female anurans showed higher helminth richness than males, considering the total sample (two localities) or one of the two localities.•The size of anurans does not influence the parasitic infections.•The findings revealed new records of Nematoda, Acanthocephala, Digenea, and Cestoda associated with host species in the Neotropical region.