Microplastics occurrence and fate in full-scale treatment wetlands

•The evaluation of two full-scale TWs, horizontal flow and floating, shows their great potential for retaining MPs efficiently.•MPs behave similarly to suspended solids, accumulating in the wetland initial zone.•The roots of vegetation play a key role in the retention of MPs.•Fibers pose the greatest challenge for MP control, despite their high removal rates. Treatment wetlands (TWs) are an efficient technology for removing microplastics (MPs) from wastewater, according to previous studies. This study investigates the dynamics and fate of MPs in two wastewater treatment plants (WWTPs) using TWs, one with horizontal subsurface flow (HF) and another with a floating plant system (FS). Special attention is paid to the retention produced in the sludge and the role of macrophyte roots. The abundance of MPs in the influent to the WWTPs was on average 20.3 ± 0.85 MP/L and 8.4 ± 1.13 MP/L in HF and FS respectively, while the effluent had 0.58 ± 0.07 MP/L and 0.17 ± 0.06 MP/L, thus giving overall efficiencies of 97.42% and 98.13%, respectively. In the HF wetland, sludge samples near the inlet and the outlet were taken, distinguishing between sludge adhered to gravel and sludge attached to roots. In the floating macrophytes, sludge samples from secondary and tertiary treatments were taken. The results indicate that roots play a significant role in MPs retention. In the HF wetland, the complex formed by roots and gravel attached more MPs than gravel alone in the final zone of the wetland. In the FS, roots retained a significant quantity of MPs, both in the secondary and tertiary treatments, thus giving rise to a sludge less concentrated in MPs. This study aims to improve the knowledge of MPs behavior and fate in full-scale TWs, providing valuable information to enhance retention efficiency. [Display omitted]