Temperature-boosted PAM-less activation of CRISPR-Cas12a combined with selective inhibitors enhances detection of SNVs with VAFs below 0.01

Temperature-boosted PAM-less activation of CRISPR-Cas12a combined with selective inhibitors enhances detection of SNVs with VAFs below 0.01

The precise identification of rare single nucleotide variations (SNVs) concomitant with excess wild-type DNA is a valuable method for minimally invasive disease diagnosis and early prediction of drug responsiveness. Selective enrichment of mutant variants via strand displacement reaction offers an i...

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Veröffentlicht in:Talanta (Oxford) 2023-08, Vol.261, p.124674-124674, Article 124674
Hauptverfasser: Chen, Kena, Dai, Ling, Zhao, Jie, Deng, Mengjun, Song, Lin, Bai, Dan, Wu, You, Zhou, Xi, Yang, Yujun, Yang, Shuangshuang, Zhao, Lin, Chen, Xueping, Xie, Guoming, Li, Junjie
Format: Artikel
Sprache:eng
Schlagworte:
Blocker displacement amplification
CRISPR-Cas Systems - genetics
CRISPR-Cas12a
Mutation
Nucleotides
PAM-Less activation
PCR additives
Point Mutation
Single nucleotide variations
Temperature
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Zusammenfassung:The precise identification of rare single nucleotide variations (SNVs) concomitant with excess wild-type DNA is a valuable method for minimally invasive disease diagnosis and early prediction of drug responsiveness. Selective enrichment of mutant variants via strand displacement reaction offers an ideal approach of SNVs analysis but fails to differentiate wildtype from mutants with variant allele fraction (VAF) 
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2023.124674