In vivo tracking of plasmid DNA/mRNA: A novel labelling precursor for 89Zr positron emission tomography

[Display omitted] Herein, we developed a novel labelling precursor Fe-DFO-5 for plasmid DNA (pDNA) utilizing 89Zr as a radioisotope for PET imaging. 89Zr-labelled pDNA showed comparable gene expression to non-labelled pDNA. The biodistribution of 89Zr-labelled pDNA after local or systemic administra...

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Veröffentlicht in:	Bioorganic & medicinal chemistry letters 2023-06, Vol.90, p.129332-129332, Article 129332
Hauptverfasser:	Aoyama, Naohiro, Kimura, Sadaaki, Matsui, Toshiyuki, Nozawa, Takashi
Format:	Artikel
Sprache:	eng
Schlagworte:	89Zr Biodistribution Bioluminescence Gene expression Gene therapy In vivo imaging mRNA PET Plasmid Positron emission tomography
Online-Zugang:	Volltext
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Zusammenfassung:	[Display omitted] Herein, we developed a novel labelling precursor Fe-DFO-5 for plasmid DNA (pDNA) utilizing 89Zr as a radioisotope for PET imaging. 89Zr-labelled pDNA showed comparable gene expression to non-labelled pDNA. The biodistribution of 89Zr-labelled pDNA after local or systemic administration in mice was evaluated. Furthermore, this labelling method was also applied to mRNA.
ISSN:	0960-894X 1464-3405
DOI:	10.1016/j.bmcl.2023.129332