Use of ultrafiltration/diafiltration for the processing of antisense oligonucleotides

Ultrafiltration/diafiltration (UF/DF) has been the hallmark for concentrating and buffer exchange of protein and peptide‐based therapeutics for years. Here we examine the capabilities and limitations of UF/DF membranes to process oligonucleotides using antisense oligonucleotides (ASOs) as a model. U...

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Veröffentlicht in:	Biotechnology progress 2023-07, Vol.39 (4), p.e3350-n/a
Hauptverfasser:	Gronke, Robert S., Ruanjaikaen, Krisada, Delavari, Armin, Immel‐Brown, Jonas P., Penrod, Joseph C., Lam, Yik, Antia, Firoz D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Antisense oligonucleotides Antisense therapy Cations Divalent cations Hybridization Impurities Magnesium membrane polarization Membranes Molecular weight oligonucleotide Oligonucleotides Robustness Ultrafiltration ultrafiltration and diafiltration
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