Use of ultrafiltration/diafiltration for the processing of antisense oligonucleotides

Use of ultrafiltration/diafiltration for the processing of antisense oligonucleotides

Ultrafiltration/diafiltration (UF/DF) has been the hallmark for concentrating and buffer exchange of protein and peptide‐based therapeutics for years. Here we examine the capabilities and limitations of UF/DF membranes to process oligonucleotides using antisense oligonucleotides (ASOs) as a model. U...

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Veröffentlicht in:Biotechnology progress 2023-07, Vol.39 (4), p.e3350-n/a
Hauptverfasser: Gronke, Robert S., Ruanjaikaen, Krisada, Delavari, Armin, Immel‐Brown, Jonas P., Penrod, Joseph C., Lam, Yik, Antia, Firoz D.
Format: Artikel
Sprache:eng
Schlagworte:
Antisense oligonucleotides
Antisense therapy
Cations
Divalent cations
Hybridization
Impurities
Magnesium
membrane polarization
Membranes
Molecular weight
oligonucleotide
Oligonucleotides
Robustness
Ultrafiltration
ultrafiltration and diafiltration
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Zusammenfassung:Ultrafiltration/diafiltration (UF/DF) has been the hallmark for concentrating and buffer exchange of protein and peptide‐based therapeutics for years. Here we examine the capabilities and limitations of UF/DF membranes to process oligonucleotides using antisense oligonucleotides (ASOs) as a model. Using a 3 kDa UF/DF membrane, oligonucleotides as small as 6 kDa are shown to have low sieving coefficients (
ISSN:8756-7938
1520-6033
DOI:10.1002/btpr.3350