Toll-like receptor expression profile of stem/progenitor cells from human exfoliated deciduous teeth
Stem/progenitor cells from human exfoliated deciduous teeth (SHED) show remarkable pluripotent, regenerative, and immunological capacities. During in vivo regenerative processes, there could be the presence of SHED in the surrounding inflammatory microenvironment, through toll-like receptors (TLRs)....
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Veröffentlicht in: | International journal of paediatric dentistry 2023-11, Vol.33 (6), p.607-614 |
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Zusammenfassung: | Stem/progenitor cells from human exfoliated deciduous teeth (SHED) show remarkable pluripotent, regenerative, and immunological capacities. During in vivo regenerative processes, there could be the presence of SHED in the surrounding inflammatory microenvironment, through toll-like receptors (TLRs).
The aim of this paper was to present a characteristic TLR expression profile on SHED for the first time.
Cells were harvested from extracted primary teeth (n = 10), anti-STRO-1 immunomagnetically sorted and cultivated, through colony-forming units (CFUs). SHED were examined for mesenchymal stem/progenitor cell traits, including the expression of clusters of differentiation (CDs) 14, 34, 45, 73, 90, 105, and 146, and their multilineage differentiation aptitude. TLRs 1-10 expression was investigated for SHED in uninflamed and inflamed (25 ng/mL IL-1β, 10
U/mL IFN-γ, 50 ng/mL TNF-α, and 3 × 10
U/mL IFN-α; SHED-i) microenvironmental conditions.
SHED were negative for CDs 14, 34, and 45, but were positive for CDs 73, 90, 105, and 146, and demonstrated characteristic multilineage differentiation. In an uninflamed microenvironment, SHED expressed TLRs 1, 2, 3, 4, 6, 8, 9, and 10. The inflammatory microenvironment downregulated TLR7 significantly on gene level and upregulated TLR8 on gene and protein levels (p |
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ISSN: | 0960-7439 1365-263X |
DOI: | 10.1111/ipd.13080 |