Disentangling extrachromosomal circular DNA heterogeneity in single cells with scEC&T-seq

Disentangling extrachromosomal circular DNA heterogeneity in single cells with scEC&T-seq

We introduce scEC&T-seq, a new single-cell sequencing method that enables parallel profiling of extrachromosomal circular DNA and mRNAs in single cells. Using scEC&T-seq, we characterized all types of circular DNA elements in single human cancer cells and profiled the intercellular heterogen...

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Veröffentlicht in:Nature genetics 2023-05, Vol.55 (5), p.740-741
Format: Artikel
Sprache:eng
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631/208/514
631/208/514/1948
Agriculture
Animal Genetics and Genomics
Biomedical and Life Sciences
Biomedicine
Cancer Research
DNA
DNA, Circular - genetics
Gene Function
Human Genetics
Research Briefing
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Beschreibung
Zusammenfassung:We introduce scEC&T-seq, a new single-cell sequencing method that enables parallel profiling of extrachromosomal circular DNA and mRNAs in single cells. Using scEC&T-seq, we characterized all types of circular DNA elements in single human cancer cells and profiled the intercellular heterogeneity and structural dynamics of cancer-specific extrachromosomal DNA.
ISSN:1061-4036
1546-1718
DOI:10.1038/s41588-023-01385-z