An industrially applicable Escherichia coli platform for bioconversion of thebaine to oripavine and codeine to morphine

A whole cell biotransformation platform converting thebaine to oripavine and codeine to morphine was demonstrated with industrially applicable yields (∼1.2 × 10 g L h or ∼1.2 × 10 g L h ), improving >13 400-fold upon morphine production in yeast. Mutations enhanced enzyme performance and the use...

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Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2023-05, Vol.59 (41), p.6251-6254
Hauptverfasser:	Spencer, Garrick W K, Li, Xu, Jarrold, Ailsa, Gras, Sally L
Format:	Artikel
Sprache:	eng
Schlagworte:	Bioconversion Biotransformation Codeine E coli Escherichia coli - genetics Escherichia coli - metabolism Morphine Substrates Thebaine - metabolism
Online-Zugang:	Volltext
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Zusammenfassung:	A whole cell biotransformation platform converting thebaine to oripavine and codeine to morphine was demonstrated with industrially applicable yields (∼1.2 × 10 g L h or ∼1.2 × 10 g L h ), improving >13 400-fold upon morphine production in yeast. Mutations enhanced enzyme performance and the use of a purified substrate with rich raw poppy extract expanded applicability.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/d3cc00534h