Sphingomicrobium sediminis sp. nov., isolated from marine sediment in the Republic of Korea

A Gram-stain-negative, rod-shaped, bright-orange coloured bacterium without flagellum, designated as strain GRR-S6-50 , was isolated from a tidal flat of Garorim bay, Taean-gun, Chungcheongbuk-do, Republic of Korea. Cells grew aerobically at 20-37 °C (optimum, 30 °C), pH 7.0-10.0 (optimum, pH 7.0) and with 1-5 % (w/v) NaCl (optimum, 3 %). The 16S rRNA gene sequence analysis demonstrated that strain GRR-S6-50 was closely related to AH-M8 with a sequence similarity of 97.80 % followed by CC-AMO-30B (97.44 %), CC- AMZ-30M (97.16 %), CAU 1457 (96.37 %), CC-AMZ-30N (95.31 %) and CC-TBT-3 (95.23 %). The average nucleotide identity and digital DNA-DNA hybridization values with related strains ranged from 74.5 to 77.3% and 21.1 to 35.0 %, respectively. The G+C content of strain GRR-S6-50 was 63.30 mol%. The strain has ubiquinone-10 as the predominant respiratory quinone and the major fatty acids were C 6 (54.57 %) and C c (10.58 %). The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, three unidentified lipids and one glycolipid. On the basis of the results of phylogenetic, phenotypic and chemotaxonomic studies, strain GRR-S6-50 is regarded to represent a novel species within the genus , for which the name sp. nov. (KACC 22562 =KCTC 92123 =JCM 35084 ) is proposed.