Heterologous expression of the lectin CmRlec from Cordyceps militaris (Cordycipitaceae, Ascomycota) in Escherichia coli

Heterologous expression of the lectin CmRlec from Cordyceps militaris (Cordycipitaceae, Ascomycota) in Escherichia coli

ABSTRACT Ascomycete lectins may play an important role in their life cycle. In this report, we mined a ricin B-type lectin, named CmRlec, from the Cordyceps militaris genome by homology search. Furthermore, we succeeded in the soluble expression of CmRlec using β-glucuronidase as a solubilization ta...

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Veröffentlicht in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2023-06, Vol.87 (7), p.742-746
Hauptverfasser: Ono, Akiko, Zhang, Jili, Tanaka, Yuki, Sato, Chihiro, Yoda, Akiyoshi, Ogata, Makoto, Nomura, Takahito, Suzuki, Tomohiro
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container_end_page 746
container_issue 7
container_start_page 742
container_title Bioscience, biotechnology, and biochemistry
container_volume 87
creator Ono, Akiko
Zhang, Jili
Tanaka, Yuki
Sato, Chihiro
Yoda, Akiyoshi
Ogata, Makoto
Nomura, Takahito
Suzuki, Tomohiro
description ABSTRACT Ascomycete lectins may play an important role in their life cycle. In this report, we mined a ricin B-type lectin, named CmRlec, from the Cordyceps militaris genome by homology search. Furthermore, we succeeded in the soluble expression of CmRlec using β-glucuronidase as a solubilization tag and demonstrated that this lectin is a novel chitin-recognizing lectin.
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source Oxford University Press Journals All Titles (1996-Current)
title Heterologous expression of the lectin CmRlec from Cordyceps militaris (Cordycipitaceae, Ascomycota) in Escherichia coli
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