Heterologous expression of the lectin CmRlec from Cordyceps militaris (Cordycipitaceae, Ascomycota) in Escherichia coli

Heterologous expression of the lectin CmRlec from Cordyceps militaris (Cordycipitaceae, Ascomycota) in Escherichia coli

ABSTRACT Ascomycete lectins may play an important role in their life cycle. In this report, we mined a ricin B-type lectin, named CmRlec, from the Cordyceps militaris genome by homology search. Furthermore, we succeeded in the soluble expression of CmRlec using β-glucuronidase as a solubilization ta...

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Veröffentlicht in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2023-06, Vol.87 (7), p.742-746
Hauptverfasser: Ono, Akiko, Zhang, Jili, Tanaka, Yuki, Sato, Chihiro, Yoda, Akiyoshi, Ogata, Makoto, Nomura, Takahito, Suzuki, Tomohiro
Format: Artikel
Sprache:eng
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Zusammenfassung:ABSTRACT Ascomycete lectins may play an important role in their life cycle. In this report, we mined a ricin B-type lectin, named CmRlec, from the Cordyceps militaris genome by homology search. Furthermore, we succeeded in the soluble expression of CmRlec using β-glucuronidase as a solubilization tag and demonstrated that this lectin is a novel chitin-recognizing lectin.
ISSN:1347-6947
1347-6947
DOI:10.1093/bbb/zbad045