Complete nucleotide sequence of hackberry virus A, a tentative member of the genus Waikavirus

The complete genomic sequence of a waikavirus from Chinese hackberry in Zhejiang province, China, named “hackberry virus A” (HVA), was determined using high-throughput sequencing (HTS) combined with reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE)...

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Veröffentlicht in:Archives of virology 2023-05, Vol.168 (5), p.137-137, Article 137
Hauptverfasser: Weng, Jiajia, Wu, Mengting, Ye, Zhuangxin, Wang, Lin, Ke, Bin, Huang, Chanchan, Lu, Yuwen, Peng, Jiejun, Lin, Lin, Rao, Shaofei, Wu, Guanwei, Chen, Jianping, Zheng, Hongying, Yan, Fei
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Sprache:eng
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Zusammenfassung:The complete genomic sequence of a waikavirus from Chinese hackberry in Zhejiang province, China, named “hackberry virus A” (HVA), was determined using high-throughput sequencing (HTS) combined with reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) PCR. The bicistronic genomic RNA of HVA was found to consist of 12,691 nucleotides (nt), excluding the 3’-terminal poly(A) tail, and to encode a large polyprotein of 3783 amino acids (aa) and an additional 10.3-kDa protein. The aa sequences of the Pro-Pol and the CP regions of this virus share 39.8–44.2% and 25.5–36.4% identity, respectively, with currently known waikaviruses. These values are significantly below the current species demarcation threshold (< 75% and < 80% aa identity for the CP and Pro-Pol region, respectively) for the family Secoviridae , indicating that HVA represents a new species in the genus Waikavirus . This is the first report of a virus infecting Chinese hackberry.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-023-05764-z