Effects of external cues and group mate body size on the collective behavior of shoaling crucian carp

Chemical alarm cues (CACs) play a key role in the predatorprey relationship in fish. Chemical cues in the aquatic environment have an impact on the individual and group behavior of fish, and differences in these behavioral responses might be related to the body size of group members. Here, we used juvenile crucian carp (Carassius carassius) as an animal model to examine the effects of different cues and group mate body sizes on the individual and group behavior of shoaling fish. Three group mate body size (small, large, and mixed size) and three pheromone (rearing tank water, food, and CACs) treatments were combined in our study, with each treatment having 16 groups of five fish. We found that the individual swimming speed of the mixed group increased after injecting rearing water and food cues in the tank. After injecting CACs, the individual swimming speed of both the small and mixed groups increased, while that of the large group did not change. After the injection of CACs, the group speed of the small group was higher than that of the large and mixed groups. After the food cues were added to the tank, the synchronization of speed of the small group was higher than that of the mixed and large groups. Both the interindividual distance and nearest-neighbor distance of the mixed group remained unchanged after injecting CACs. Our study indicated that the impact of external cues on the individual and collective behavior of fish is related to the difference in body size of group mates. •The injection of both the rearing tank water (control) and food cues increased the individual swimming speed in the mixed fish group.•The individual swimming speed of the mixed fish group decreased after the injection of the CACs.•After the CACs were injected, the group speed of the small fish group was higher than that of the other two groups.•The group cohesion is higher in the mixed fish group than in the other two groups.