Acute impact of salinity and C/N ratio on the formation and properties of soluble microbial products from activated sludge

The present study investigated the shock of NaCl and C/N ratio on properties of soluble microbial products (SMPs), focusing on their sized fractions. The results indicated that the NaCl stress increased the content of biopolymers, humic substances, building blocks, and LMW substances in SMPs, while the addition of 40 g NaCl L−1 significantly changed their relative abundance in SMPs. The acute impact of both N-rich and N-deficient conditions accelerated the secretion of SMPs, but the characteristics of LMW substances differed. Meanwhile, the bio-utilization of SMPs has been enhanced with the increase of NaCl dosage but decreased with the increase of the C/N ratio. The mass balance of sized fractions in SMPs + EPS could be set up when NaCl dosage 5, which indicates the hydrolysis of sized fractions in EPS mainly compensated for their increase/reduction in SMPs. Besides, the results of the toxic assessment indicated that the oxidative damage caused by the NaCl shock was an important factor affecting the property of SMPs, and the abnormal expression of DNA transcription cannot be neglected for bacteria metabolisms with the change of C/N ratio. [Display omitted] •The formation of sized fractions had been altered by both NaCl and C/N ratio shock.•The bio-utilization level of SMPs has been changed by both NaCl and C/N ratio shock.•The hydrolysis of EPS was the main reason for the SMPs increase/reduction.•Serious oxidative damage in cells occurred under the shock of 40 g NaCl L−1.•Shock of the C/N ratio at 2 caused obvious abnormal expression of DNA transcription.