The influence of pure (ligandless) magnetite nanoparticles functionalization on blood gases and electrolytes in acute blood loss

Objective was to compare the effect of functionalization of magnetite (Fe3O4) nanoparticles (NPs) with sodium chloride (NaCl), or its combination with ethylmethylhydroxypyrydine succinate (EMHPS) and polyvinylpyrrolidone (PVP) on blood gases and electrolytes in acute blood loss. Ligandless magnetite NPs were synthesized by the electron beam technology and functionalized by mentioned agents. Size of NPs in colloidal solutions Fe3O4@NaCl, Fe3O4@NaCl@EMHPS, Fe3O4@NaCl@PVP, Fe3O4@NaCl@EMHPS@PVP (nanosystems 1–4) was determined by dynamic light scattering. In vivo experiments were performed on 27 Wistar rats. Acute blood loss was modeled by removal 25 % circulating blood. Nanosystems 1–4 were administered to animals intaperitoneally after the blood loss with followed determination of blood gases, pH and electrolytes. In blood loss, nanosystems Fe3O4@NaCl and Fe3O4@NaCl@PVP were able to improve the state of blood gases, pH, and the ratio of sodium/potassium in the blood. So, magnetite NPs with a certain surface modification can promote oxygen transport under hypoxic conditions. Electron-beam physical vapor deposition synthesis of pure (ligandless) magnetite nanoparticles in a sodium chloride matrix is presented. When modifying the surface with salt, magnetite nanoparticles can promote oxygen transport, elevate hydrogen index, and maintain sodium/potassium balance in the blood under conditions of acute blood loss. Magnetite nanoparticles with polyvinylpyrrolidone increase hemoglobin saturation by oxygen, volumetric concentration of oxygen and hydrogen index in the blood of laboratory animals in the blood loss. This opens up prospects to use magnetite nanoparticles with certain coatings to treat hypoxic conditions. [Display omitted] •Electron-beam physical synthesis of ligandless magnetite nanoparticles in a sodium chloride matrix is presented•When modifying the surface with salt, magnetite nanoparticles can promote oxygen transport and maintain electrolyte balance in blood loss•Magnetite nanoparticles with polyvinylpyrrolidone increase hemoglobin saturation by oxygen and hydrogen index in the blood