Flavobacterium frigoritolerans sp. nov. and Flavobacterium shii sp. nov., isolated from glaciers on the Tibetan Plateau

The genus belongs to the family and its members are widely distributed in the environment. Taxonomic descriptions of strains LS1R47 and LS1R49 isolated from the Laigu glacier on the Tibetan Plateau, China, are presented in this study. Both strains were psychrotolerant, Gram-stain-negative, aerobic and rod-shaped. The comparative analysis of 16S rRNA gene sequences showed that strain LS1R47 was closest to CIP 105534 (98.90 %) and strain LS1R49 was closest to 983-08 (98.73 %). The 16S rRNA gene sequence similarity, average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the two novel isolates were 99.4, 86.0 and 30.9 %, respectively. The ANI and dDDH values between strains LS1R47 and LS1R49 and their closely relatives were below 87.6 and 33.3 %, respectively. Phylogenomic analysis showed that the two strains cluster together with ATCC 29551 . Both strains contained MK-6 as sole quinone, phosphatidylethanolamine as the principal polar lipid, and summed feature 3 (iso-C 2-OH and/or C ω7c), iso-C 3-OH, C 3-OH and iso-C 3-OH as the main fatty acids. These results indicated that strains LS1R47 and LS1R49 represented two novel species within the genus . Therefore, we propose two novel species, sp. nov. (LS1R47 =CGMCC 1.11577 =NBRC 113654 ) and sp. nov. (LS1R49 =CGMCC 1.11581 =NBRC 113652 ).