Fiber SPR biosensor sensitized by MOFs for MUC1 protein detection

The concentration of tumor markers is low, which needs a highly sensitive, stable and fast detection method. In this paper, we proposed and demonstrated a U-shape fiber SPR biosensor sensitized by MOFs materials. The surface of the U-shape SPR sensor was modified with MOFs materials to enhance the sensitivity, and the nucleic acid aptamer was immobilized on the sensor surface because of the biocompatibility of MOFs materials. By the high specificity of the nucleic acid aptamer, the MUC1 protein was recognized and detected. The testing results indicate that the sensor has a logarithmic linear response in the MUC1 protein concentration detection range of 1 pg/ml-100 μg/ml, its sensitivity and detection limit are 5.33 nm/log(μg/ml) and 0.16 pg/ml respectively. After being sensitized by MOFs, the detection sensitivity of the sensor can be increased by 1.62 times，the LOD can be decreased by 0.75 times. The sensor has high sensitivity and specificity, which has broad application prospects in clinical detection of tumor markers. [Display omitted] •The fiber SPR sensing probe was realized by using a U-shaped structure which was easy to insert into a narrow space.•The surface of the U-shape SPR sensor was modified with MOFs materials to enhance the sensitivity.•The nucleic acid aptamer was immobilized on the surface of the U-shaped fiber probe to detect the low concentration MUC1 protein.•By combining the U-shaped fiber SPR sensor with other nucleic acid aptamer, other types of tumor markers can be detected.