Convalescent action of menthol against T-2 mycotoxin-induced toxicity: An in vitro study with HaCaT cells

Only T-2 mycotoxin is emitted as an aerosol and is the most toxic fungal secondary metabolite among mycotoxins. In its clinical condition, the skin is severely irritated and painful due to lesions and alimentary toxic aleukia. Herein, we have assessed various bioactive molecules, viz. kaempferol, menthol, curcumin, and quercetin, against T-2-induced toxicity in HaCaT cells. Menthol offered exceptional protection, protecting 92% of HaCaT cells after exposure to 300 nM T-2 and reducing LDH leakage by up to 42%. Its pre-treatment provided considerable protection against T-2 toxicity, as evidenced by the assessment of mitochondrial membrane potential. Propidium iodide staining revealed a cell cycle halt at the G1, S, and M phases and a significant increase in the sub-G1 percentage in T-2-challenged cells, indicating cell death. However, pre-treatment with menthol promoted cell cycle progression in cells exposed to T-2. Immunoblotting results demonstrated that menthol resulted in a discernible down-regulation of i-NOS expression in T-2-challenged HaCaT cells. •T-2-induced toxicity in HaCaT (Keratinocytes) cells-In vitro study.•Screening of Phytochemicals for the amelioration of T-2 toxin induced dermal toxicity.•Identification of Menthol Convalescent action against T-2 toxin induced toxicity in HaCaT cells.•MMP and ROS estimation was evaluated to test the efficacy of menthol against T-2 induced toxicity.•Flow cytometry and immunoblotting methods were used to confirm the activity of Menthol.