Preparation and chromatographic performance of chiral peptide‐based stationary phases for enantiomeric separation

This study presents the development of three new chiral stationary phases. They are based on silica modified with peptides containing phenylalanine and proline. Successful analyses and characterizations were conducted using Fourier transform infrared spectra, elemental analysis, and thermogravimetric analysis. After this, the enantioselective performance of the three chiral peptide‐based columns was evaluated. The evaluation used 11 racemic compounds under normal‐phase high performance liquid chromatography mode. Optimized enantiomeric separation conditions were established. Under these conditions, the enantiomers of flurbiprofen and naproxen were successfully separated on CSP‐1 column: the separation factor of these was 1.27 and 1.21, respectively. In addition, the reproducibility of the CSP‐1 column was also investigated. The results of the investigation illustrated that the stationary phases have good reproducibility (RSD = 0.73%, n = 5). Preparation of three different chiral stationary phases (CSPs): based on D‐ or L‐type amino acid derivatives bonded to modified silica. Three CSPs were utilized for the separation of enantiomeric separation. Several main factors affecting the enantioseparation performance on three CSPs were investigated.