Focal adhesion signaling pathway involved in skin immune response of tongue sole Cynoglossus semilaevis to Vibrio vulnificus infection
Focal adhesion (FA) plays a key role in cell adhesion, migration and antibacterial immune, but it remained unclear in fish. In this study, half-smooth tongue sole Cynoglossus semilaevis were infected with Vibrio vulnificus, and then immune-related protein in the skin, especially for FA signaling pat...
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| Veröffentlicht in: | Fish & shellfish immunology 2023-04, Vol.135, p.108651-108651, Article 108651 |
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| container_title | Fish & shellfish immunology |
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| creator | Liu, Houfu Hu, Xiucai Lian, Zhengyi Luo, Zhang Lv, Aijun Tan, Jing |
| description | Focal adhesion (FA) plays a key role in cell adhesion, migration and antibacterial immune, but it remained unclear in fish. In this study, half-smooth tongue sole Cynoglossus semilaevis were infected with Vibrio vulnificus, and then immune-related protein in the skin, especially for FA signaling pathway were screened and identified by iTRAQ analysis. Results showed that the differentially expressed proteins (DEPs) in skin immune response (eg., ITGA6, FN, COCH, AMBP, COL6A1, COL6A3, COL6A6, LAMB1, LAMC1, FLMNA) were firstly found in FA signaling pathway. Furthermore, the validation analysis of FA-related genes were basically consistent with the iTRAQ data at 36 hpi (r = 0.678, p |
| doi_str_mv | 10.1016/j.fsi.2023.108651 |
| format | Article |
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•FA signaling pathway in C. semilaevis upon V. vulnificus were screened and identified by iTRAQ analysis.•Some DEPs in skin immune response were firstly found in FA signaling pathway.•The spatio-temporal expression of FA-related genes was confirmed by qPCR analysis.</description><identifier>ISSN: 1050-4648</identifier><identifier>EISSN: 1095-9947</identifier><identifier>DOI: 10.1016/j.fsi.2023.108651</identifier><identifier>PMID: 36863497</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Cell Adhesion ; Cynoglossus semilaevis ; Fish Diseases ; Fish Proteins ; Flatfishes ; Flounder - metabolism ; Focal adhesion ; Focal Adhesions - metabolism ; Immunity ; Vibrio - physiology ; Vibrio Infections ; Vibrio vulnificus ; Vinculin</subject><ispartof>Fish & shellfish immunology, 2023-04, Vol.135, p.108651-108651, Article 108651</ispartof><rights>2023</rights><rights>Copyright © 2023. Published by Elsevier Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-f91fc5fd5c106810db57b8fcb61f0b11d5d6c0b25219b25aa1c2278af24dce0a3</citedby><cites>FETCH-LOGICAL-c353t-f91fc5fd5c106810db57b8fcb61f0b11d5d6c0b25219b25aa1c2278af24dce0a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.fsi.2023.108651$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27911,27912,45982</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36863497$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Houfu</creatorcontrib><creatorcontrib>Hu, Xiucai</creatorcontrib><creatorcontrib>Lian, Zhengyi</creatorcontrib><creatorcontrib>Luo, Zhang</creatorcontrib><creatorcontrib>Lv, Aijun</creatorcontrib><creatorcontrib>Tan, Jing</creatorcontrib><title>Focal adhesion signaling pathway involved in skin immune response of tongue sole Cynoglossus semilaevis to Vibrio vulnificus infection</title><title>Fish & shellfish immunology</title><addtitle>Fish Shellfish Immunol</addtitle><description>Focal adhesion (FA) plays a key role in cell adhesion, migration and antibacterial immune, but it remained unclear in fish. In this study, half-smooth tongue sole Cynoglossus semilaevis were infected with Vibrio vulnificus, and then immune-related protein in the skin, especially for FA signaling pathway were screened and identified by iTRAQ analysis. Results showed that the differentially expressed proteins (DEPs) in skin immune response (eg., ITGA6, FN, COCH, AMBP, COL6A1, COL6A3, COL6A6, LAMB1, LAMC1, FLMNA) were firstly found in FA signaling pathway. Furthermore, the validation analysis of FA-related genes were basically consistent with the iTRAQ data at 36 hpi (r = 0.678, p < 0.01), and their spatio-temporal expressions were confirmed by qPCR analysis. The molecular characterization of vinculin of C. semilaevis was described. This study will provide a new perspective for understanding the molecular mechanism of FA signaling pathway in the skin immune response in marine fish.
•FA signaling pathway in C. semilaevis upon V. vulnificus were screened and identified by iTRAQ analysis.•Some DEPs in skin immune response were firstly found in FA signaling pathway.•The spatio-temporal expression of FA-related genes was confirmed by qPCR analysis.</description><subject>Animals</subject><subject>Cell Adhesion</subject><subject>Cynoglossus semilaevis</subject><subject>Fish Diseases</subject><subject>Fish Proteins</subject><subject>Flatfishes</subject><subject>Flounder - metabolism</subject><subject>Focal adhesion</subject><subject>Focal Adhesions - metabolism</subject><subject>Immunity</subject><subject>Vibrio - physiology</subject><subject>Vibrio Infections</subject><subject>Vibrio vulnificus</subject><subject>Vinculin</subject><issn>1050-4648</issn><issn>1095-9947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1v1DAQhi0EomXhB3BBPnLJYufDm4gTWrWlUqVeClfLscfbWRx7ySRB-wf6u_FqC8de5kPzzjuah7GPUqylkOrLfu0J16Uoq9y3qpGv2KUUXVN0Xb15faobUdSqbi_YO6K9EEJVSrxlF5VqVVV3m0v2dJ2sCdy4RyBMkRPuogkYd_xgpsc_5sgxLiks4HLB6VcOOAxzBD4CHVIk4MnzKcXdDJxSAL49xrQLiWgmTjBgMLAgZQn_if2IiS9ziOjR5jlGD3bKd9-zN94Egg_PecV-XF89bL8Xd_c3t9tvd4WtmmoqfCe9bbxrrBSqlcL1zaZvve2V9KKX0jVOWdGXTSm7HI2Rtiw3rfFl7SwIU63Y57PvYUy_Z6BJD0gWQjAR0kw6izOXSuZrKybPUjvmZ0bw-jDiYMajlkKf8Ou9zvj1Cb8-4887n57t534A93_jH-8s-HoWQH5yQRg1WYRoweGYSWiX8AX7v9-cmWY</recordid><startdate>202304</startdate><enddate>202304</enddate><creator>Liu, Houfu</creator><creator>Hu, Xiucai</creator><creator>Lian, Zhengyi</creator><creator>Luo, Zhang</creator><creator>Lv, Aijun</creator><creator>Tan, Jing</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202304</creationdate><title>Focal adhesion signaling pathway involved in skin immune response of tongue sole Cynoglossus semilaevis to Vibrio vulnificus infection</title><author>Liu, Houfu ; Hu, Xiucai ; Lian, Zhengyi ; Luo, Zhang ; Lv, Aijun ; Tan, Jing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-f91fc5fd5c106810db57b8fcb61f0b11d5d6c0b25219b25aa1c2278af24dce0a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Cell Adhesion</topic><topic>Cynoglossus semilaevis</topic><topic>Fish Diseases</topic><topic>Fish Proteins</topic><topic>Flatfishes</topic><topic>Flounder - metabolism</topic><topic>Focal adhesion</topic><topic>Focal Adhesions - metabolism</topic><topic>Immunity</topic><topic>Vibrio - physiology</topic><topic>Vibrio Infections</topic><topic>Vibrio vulnificus</topic><topic>Vinculin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Houfu</creatorcontrib><creatorcontrib>Hu, Xiucai</creatorcontrib><creatorcontrib>Lian, Zhengyi</creatorcontrib><creatorcontrib>Luo, Zhang</creatorcontrib><creatorcontrib>Lv, Aijun</creatorcontrib><creatorcontrib>Tan, Jing</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fish & shellfish immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Houfu</au><au>Hu, Xiucai</au><au>Lian, Zhengyi</au><au>Luo, Zhang</au><au>Lv, Aijun</au><au>Tan, Jing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Focal adhesion signaling pathway involved in skin immune response of tongue sole Cynoglossus semilaevis to Vibrio vulnificus infection</atitle><jtitle>Fish & shellfish immunology</jtitle><addtitle>Fish Shellfish Immunol</addtitle><date>2023-04</date><risdate>2023</risdate><volume>135</volume><spage>108651</spage><epage>108651</epage><pages>108651-108651</pages><artnum>108651</artnum><issn>1050-4648</issn><eissn>1095-9947</eissn><abstract>Focal adhesion (FA) plays a key role in cell adhesion, migration and antibacterial immune, but it remained unclear in fish. In this study, half-smooth tongue sole Cynoglossus semilaevis were infected with Vibrio vulnificus, and then immune-related protein in the skin, especially for FA signaling pathway were screened and identified by iTRAQ analysis. Results showed that the differentially expressed proteins (DEPs) in skin immune response (eg., ITGA6, FN, COCH, AMBP, COL6A1, COL6A3, COL6A6, LAMB1, LAMC1, FLMNA) were firstly found in FA signaling pathway. Furthermore, the validation analysis of FA-related genes were basically consistent with the iTRAQ data at 36 hpi (r = 0.678, p < 0.01), and their spatio-temporal expressions were confirmed by qPCR analysis. The molecular characterization of vinculin of C. semilaevis was described. This study will provide a new perspective for understanding the molecular mechanism of FA signaling pathway in the skin immune response in marine fish.
•FA signaling pathway in C. semilaevis upon V. vulnificus were screened and identified by iTRAQ analysis.•Some DEPs in skin immune response were firstly found in FA signaling pathway.•The spatio-temporal expression of FA-related genes was confirmed by qPCR analysis.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>36863497</pmid><doi>10.1016/j.fsi.2023.108651</doi><tpages>1</tpages></addata></record> |
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| subjects | Animals Cell Adhesion Cynoglossus semilaevis Fish Diseases Fish Proteins Flatfishes Flounder - metabolism Focal adhesion Focal Adhesions - metabolism Immunity Vibrio - physiology Vibrio Infections Vibrio vulnificus Vinculin |
| title | Focal adhesion signaling pathway involved in skin immune response of tongue sole Cynoglossus semilaevis to Vibrio vulnificus infection |
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